Methods Airborne fungi were isolated and identified by using gravitational precipitation method ( sheet glass and culture plate of exposure) determination. 方法采用空气中曝片调查法和曝皿调查法,观察气传真菌的品种和数量;
A Counting Method for Monolayer Cells Attached to Culture Plate in Situ 培养板单层贴壁细胞的原位计数方法
Another 24-well culture plate was used, each well was added by 1 mL monocyte suspension. 另取两个24孔培养板,每孔只加单核细胞悬液1mL。
Methods Cell suspension obtained from ventral mesencephalon of new born rat ( 1 day) inoculated in 24 pore culture plate. 方法取1d的新生大鼠中脑腹侧细胞悬液,将其接种于24孔培养板中进行培养。
All cells and small tissues were planted in 12-well culture plate and the culture cells morphological characteristics were observed by Wright staining during the various time periods. 细胞接种于12孔培养板,并于培养不同时间行Wright染色检查细胞生长情况。
Bladder epithelial cells proliferated in vitro were inoculated to normal culture plate ( Control group) and fibrin glue-coated culture plate ( Experimental group) at the same density. Cellular clone was compared between control group and experimental group 4 days after inoculation. 将体外扩增的膀胱上皮细胞以相同密度分别接种于普通培养板(对照组)和纤维蛋白凝胶包被的培养板(实验组),于接种后4d比较两组细胞克隆形成情况。
The cells cultured on the bottom of culture plate were stretched according to the deformation of the elastic bottom. 通过真空作用使培养板底壁的弹性膜发生变形,从而使培养在其上的细胞受到拉伸力作用。
After two weeks subculture, the cells can overspread the culture plate, most of which look like shuttle. 传代乙酞化壳多糖膜的制备、性质及其生物相容性的研究培养两周后细胞铺满整个培养板孔,大部分细胞呈梭形。
Two weeks later, the cells can overspread the culture plate, which looks like spindle. 培养一周后,细胞生长旺盛,两周后细胞铺满整个培养板孔,呈纺锤形。
Results: Cells in common culture plate were mainly adhere at the side contacted to culture plate and few in the PHA. 结果:普通培养板中细胞主要黏附在PHA与培养板相接触的一侧,在载体的内部难以见到细胞分布。
On the basis of this frequency, a proper dose of phage was inoculated into 96 hole cell culture plate and was amplified by bacteria culture. 根据测得的频率,将适量的噬菌体接种于96孔细胞培养板上进行细菌培养扩增。
Conclusion It is a good special, sensitive and exact test method by Hela cell culture plate. 结论:用Hela细胞培养片为底物检测ANA及ACA是一种特异、敏感、准确的实验方法。
Conclusion As to cell culture plate and enzyme label plate, both have same diameter, flat bottom and high transparency, therefore they can be used as the special vessel for the upside-down microscopy analyzing system of urine sediment. 结论孔底直径一致、底面平整且透光性好的细胞培养板和酶标板都可以作为尿沉渣倒置显微镜分析系统的专用尿沉淀板。
Treat cell culture plate with the extracted FN, observe the growth of cells and compare with that on imported culture plate. 用纯化的牛FN处理细胞培养板,观察细胞在板中的生长情况,并与在进口板中的生长情况进行比较。
Mesenchymal stem cells ( MSCs) can be isolated from various adult tissues of human by their ability of adhering plastic culture plate wall. 间充质干细胞(MesenchymalStemcells,MSCs)存在于成人体内许多组织内,可以通过其贴壁能力分离出来。
Above-mentioned culture plate was put in the incubator for 4 hours, and then changed into 3 mL normal culture solution for 2 hours. 将以上培养板置入培养箱内培养4h,然后更换正常培养液3mL,培养2h。
Objective In order to develop a satisfactory vessel to precipitate urine sediment, compared cell culture plate with enzyme label plate, which are used in urine sediment detection by upside-down microscopy. 目的对倒置显微镜法检查尿沉渣使用的细胞培养板和酶标板进行比较,以寻找适合作为专用尿沉淀板的容器。
The Application of Tissue Culture Plate in Microalgae Collection 组织培养板在获取微藻种质中的应用
The 6-hour cell adhesion rate was 31.6%, higher than normal culture plate by statistic. 细胞在材料表面贴附良好,6小时贴壁率为31.6%,经统计学比较优于普通培养板。
This method was carried on 96-well cell culture plate, quantifying the half virus replication reduction by quantifying GFP was used to replace quantifying the half CPE reduction by cell dyeing. 此方法在96孔细胞培养板上进行操作,通过GFP检测定量半数病毒复制抑制来替代通过细胞染色定量半数CPE抑制。
Cornea epithelial cell of rabbits was cultured respectively on the membrane and the normal culture plate as a control group. MTT assay was used to detect the 6-hour cell adhesion rates and a statistical comparison was carried out between the two groups. 在材料上接种兔角膜上皮细胞,并以普通培养板为对照组,MTT法分别测定两组6小时细胞贴壁率并进行统计学比较。
To be adherent growth of MSCs, will be under sterile conditions, isolated rat preantral follicles moved to culture plate. 待MSCs贴壁生长后,将无菌条件下分离的大鼠窦前卵泡移入培养板内。
Furthermore cells were add to 24-well culture plate ( 106 cells per well) and treated as above mentioned. 另在24孔板中每孔加入106细胞,分组及处理同上。